The total number of registered companies in the county now comes to 43,528 and during 2016 6,271 new companies were established across Derbyshire.
The statistics come from the Inform Direct Review of UK Company Formations, using data from Companies House and the Office of National Statistics.
Derby formed the most new businesses, with 2,349, this was followed by Erewash, with 589, and Chesterfield, with 549.
Of the total number of new firms formed in Derbyshire 532 were in the High Peak, 388 in North East Derbyshire, 549 in Chesterfield, 472 in Bolsover, 516 in Amber Valley and 375 in Derbyshire Dales.
John Korchak, director of operations at Inform Direct said: “These figures underline the positive entrepreneurial culture that fosters new company formations and drives economic growth.
“So far, at least, we’re not seeing evidence that the Brexit vote has dented confidence among those considering starting up in business.
“Derbyshire again delivered an impressive number of new companies in 2016, demonstrating that it offers a fertile environment for new businesses to spring up and prosper.”
Across the UK as a whole, there were a record-breaking 664,720 companies formed – up from 612,565 in 2015.
The total number of UK companies now stands at 3,962,909.
Of the 601,418 formations in England, 209,869 were in London.
There were 35,502 companies formed in Scotland, while 15,795 were formed in Wales and 7,389 formed in Northern Ireland.