The incident, which happened at about 4pm on Tuesday, involved a motorbike, a car and a lorry.
Fire crews from Matlock, Wirksworth and Staveley rescued the motorcyclist, who ended up underneath the lorry, and the driver of the car who was trapped in his vehicle.
The motorcyclist, 50, of Belper, was taken to Queen’s Medical Centre in Nottingham with serious injuries. The driver was also admitted to hospital with minor injuries.
A spokesman for Derbyshire police this morning said the casualties remain in hospital.
Police shut Buxton road after diggers report bomb discovery
14 photos show parched fields, dwindling rivers and barren reservoirs across Derbyshire – as drought is officially declared
Buxton mum and daughter donate hair to Little Princess Trust cancer charity
Shocking CCTV footage shows moment man smashes glass into another man's face in Buxton pub
Buxton dad targets Three Peaks in 24 hours to help fellow cancer patients
Police and the air ambulance also attended the incident on the notorious road, which was closed until about 10pm.