Dame Sarah Storey took her total of Paralympic gold medals to 13 after a dominant performance in the time trial C5 on Wednesday.
Storey finished over a minute-and-a-half clear of her closest rivals to claim her second gold medal of Rio 2016, following victory in the 3km pursuit C5 on the track on Thursday.
The 38-year-old finished in a time of 27:22.42 with Poland’s Anna Harkowska second and America’s Samantha Bosco third.
The victory was ParalympicsGB’s 35th gold medal in Rio, taking them clear of the tally achieved at London 2012.
Storey, who still has the road race C4-5 event to come this weekend, said: “It was just about getting a really solid start.
“It was a tail wind on the way out so that was a quick section and it was just about being sensible and pushing on.
“I wasn’t 100 per cent sure I had won, your time checks are good but until they announce it officially you never know.
“You focus on one race at a time, for me it was about getting a second gold medal at these Games.
“Every day in training I’m pushing on. I keep pushing myself in the knowledge my rivals are going to catch me and thankfully it’s not yet.
“I will do the data crunching when I get home and see where we think we can head going forwards.”
The Disley rider started the Games in world record breaking fashion when she became the most decorated British female Paralympian after claiming her 12th gold medal.
Swimmer-turned-cyclist Storey - at her seventh Games - surpassed the medal haul of former wheelchair racer Baroness Tanni Grey-Thompson on Thursday night.
It was a dominant victory for Storey on the opening day of the Games over GB team-mate Crystal Lane.
She had already set a world record time in qualifying in 3:31.394 - more than 17 seconds quicker than Lane.
In the final she overtook Lane before the halfway point to claim gold.
Lane, appearing in her second Paralympics, won her first medal with silver.
“Gold medal number 12. I never thought that was possible,” said Storey, who won her first two golds as a swimmer at the 1992 Games in Barcelona.
“After London everyone expected I might retire. Then I had a baby and everyone definitely thought I was going to retire.
“I’ve gone faster today. The gap to my opposition is bigger. I didn’t expect that at all.”
Storey narrowly missed out on a medal in the C4-5 500m time trial but remained upbeat ahead of Wednesday’s race.
She will look to make it a hat-trick of medals when she rides in the C4-5 road race on Saturday.
- Stay up to the minute with her progress in Brazil at www.buxtonadvertiser.co.uk/sport.